Chemokine-like receptor 1 expression by macrophages in vivo: regulation by TGF-beta and TLR ligands.

Exp Hematol Aug , 2006

  • Brian A Zabel
  • Takao Ohyama
  • Luis Zuniga
  • Ji-Yun Kim
  • Brent Johnston
  • Samantha J Allen
  • David G Guido
  • Tracy M Handel
  • Eugene C Butcher

Chemokine-like receptor 1 (CMKLR1) is expressed by human antigen presenting cells and binds to chemerin, a proteolytically activatable chemoattractant. Here we assessed the expression of mCMKLR1 on mouse leukocytes, focusing on ex vivo dendritic cells (DC) and macrophages. mCMKLR1-expressing cells were evaluated for functional responses to chemerin. We examined the regulation of mCMKLR1 expression by exposure to toll-like receptor (TLR) ligands and cytokines. Finally, we evaluated ex vivo human ascites macrophages for huCMKLR1 expression and chemerin responsiveness.


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