Chemokine-like receptor 1 expression by macrophages in vivo: regulation by TGF-beta and TLR ligands.
Exp Hematol Aug ,
2006
Author(s):
Brian A Zabel
Takao Ohyama
Luis Zuniga
Ji-Yun Kim
Brent Johnston
Samantha J Allen
David G Guido
Tracy M Handel
Eugene C Butcher
Abstract:
Chemokine-like receptor 1 (CMKLR1) is expressed by human antigen presenting cells and binds to chemerin, a proteolytically activatable chemoattractant. Here we assessed the expression of mCMKLR1 on mouse leukocytes, focusing on ex vivo dendritic cells (DC) and macrophages. mCMKLR1-expressing cells were evaluated for functional responses to chemerin. We examined the regulation of mCMKLR1 expression by exposure to toll-like receptor (TLR) ligands and cytokines. Finally, we evaluated ex vivo human ascites macrophages for huCMKLR1 expression and chemerin responsiveness.
