Robust expansion of dendritic cells in vivo by hydrodynamic FLT3L-FC gene transfer.

J Immunol Methods Nov , 2014

  • Hua Tu
  • Thomas M Burke
  • Cecilia Oderup
  • Kexin Huang
  • Kathryn Wong
  • Susanna Lewén
  • Melissa LaJevic
  • Brian A Zabel

Due to low numbers of endogenous dendritic cells (DCs) in vivo, exogenous DC-poietin Fms-like tyrosine kinase 3-ligand (FLT3L) is routinely used to generate DC for subsequent studies. We engineered a novel FLT3L-FC DNA construct that, when combined with hydrodynamic gene transfer (HDT), induced robust DC expansion in mice. DC generated in vivo by FLT3L-FC HDT produced cytokines in response to stimulation by an array of TLR agonists and promoted T cell proliferation. The FLT3L-FC protein produced in vivo spontaneously homodimerized to enable effective FLT signaling and the FC-domain enhanced its plasma half-life, providing an improved reagent and method to boost DC numbers.


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